A combinatorial histidine scanning library approach to engineer highly pH-dependent protein switches
نویسندگان
چکیده
منابع مشابه
A generic approach to engineer antibody pH-switches using combinatorial histidine scanning libraries and yeast display
There is growing interest in the fast and robust engineering of protein pH-sensitivity that aims to reduce binding at acidic pH, compared to neutral pH. Here, we describe a novel strategy for the incorporation of pH-sensitive antigen binding functions into antibody variable domains using combinatorial histidine scanning libraries and yeast surface display. The strategy allows simultaneous scree...
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In this paper, we propose an algorithm for the design of lead generation libraries required in combinatorial drug discovery. This algorithm addresses simultaneously the two key criteria of diversity and representativeness of compounds in the resulting library and is computationally efficient when applied to a large class of lead generation design problems. At the same time, additional constrain...
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BACKGROUND In antibody purification processes, the acidic buffer commonly used to elute the bound antibodies during conventional affinity chromatograph, can damage the antibody. Herein we describe the development of several types of affinity ligands which enable the purification of antibodies under much milder conditions. RESULTS Staphylococcal protein A variants were engineered by using both...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2011
ISSN: 0961-8368
DOI: 10.1002/pro.696